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Description
Rat NT-3 (Neurotrophin 3) ELISA KitThe Rat (NT 3) Neurotrophin 3 ELISA Kit measures Neurotrophin 3 in Rat samples. The plate has been pre coated with Rat NT 3 antibody. NT 3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT 3 Antibody is added and binds to NT 3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NT 3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Rat (NT-3) Neurotrophin 3 ELISA Kit measures Neurotrophin 3 in Rat samples. The plate has been pre-coated with Rat NT-3 antibody. NT-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT-3 Antibody is added and binds to NT-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NT-3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NT-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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